BACTERIAS DEGRADADORAS DE HIDROCARBUROS AISLADAS EN SUELOS CONTAMINADOS DE PETROLEO Aislar cepas bacterianas y. Aislamiento de bacterias potencialmente degradadoras de petróleo en hábitats de ecosistemas costeros en la Bahía de Cartagena, Colombia. Download Citation on ResearchGate | SELECCIÓN DE BACTERIAS CON CAPACIDAD DEGRADADORA DE HIDROCARBUROS Estudio y selección de bacterias aerobias degradadoras de hidrocarburos del petróleo aisladas de costas.

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Enhanced biodegradation of Casablanca crude oil by a microbial consortium in presence of a rhamnolipid produced by Pseudomonas aeruginosa AT Some authors place some species of this genus into Alteromonas instead [17].

Universidad de Oriente; Pure cultures of these strains were further used in crude oil biodegradability assays. These compounds did not mineralize completely, accumulating instead as intermediary metabolites consisting of linear chains and aromatic compounds. Isolation and characterization of polycyclic aromatic hydrocarbon-degrading bacteria associated with the rhizosphere of salt marsh plants.

Strain F10S1 degraded After concluding the three subculture cycles, 0. Robust hydrocarbon degradation and dynamics of bacterial communities during nutrient-enhanced oil spill bioremediation. Mechanisms for solvent tolerance in bacteria. A Mycobacterium strain vacterias extended capacities for degradation of gasoline hydrocarbons.

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J Ind Microbiol Biot. An analysis of these results leads us to suppose that linear chains up to 30 carbon atoms long and some low molecular weight aromatic compounds were degraded during the first 10 to 15 days. F10S1 and Bacillus sp. Three replicates were seeded per strain. Effects of rhamnolipid biosurfactants on removal of phenanthrene from soil. Microbiol Mol Biol Rev.

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Appl Environ Microbiol ;63 9: Prentice Hall, Iberia; They were taxonomically identified as members of the Bacillus, Alcaligenes, Pseudomonas, Acinetobacter, Marinomonas, Kurthia and Azotobacter genera.

Impact of irradiation and polycyclic aromatic hydrocarbon spiking on microbial populations in marine sediment for future aging and biodegradability studies. Culturing these strains in the presence of hydrocarbons Mesa 30 crude favored ppetroleo expression of enzyme systems involved in their degradation and the preferential isolation of hydrocarbon-tolerant clones. Microbial degradation of hydrocarbons in the environment.


Characterization of the hydrocarbon degradation capacity of the selected strains The ability to degrade hydrocarbons from crude oil Mesa 30 crude was determined after 45 days of petroldo culture. Finer taxonomic classification to the level of species was not pursued since, despite the existence of taxonomic identification schemes for microorganisms from marine ecosystems [12, 15], successfully identifying recently described genera and species of marine bacteria requires the application of molecular methods [16].

Another two strains belonged to the genera Acinetobacter and Marinomonasrespectively. When comparing the degradation of asphaltenes with that of saturated and aromatic hydrocarbons regarding the time point at which they first become detectable as well as their kinetics, it is useful to take into account the environmental characteristics of the habitat from which hacterias microorganisms under examination have been isolated; that is, their environmental adaptations. Asphaltenes were precipitated with n-pentane.

Bacterial community dynamics and polycyclic aromatic hydrocarbon degradation during bioremediation of heavily creosote-contaminated soil. Simultaneous degradation of atrazine and phenol by Pseudomonas sp.

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Aliphatic hydrocarbons decreased deggadadoras comparison with those of the abiotic control regarding the non-resolved background cycloalkanes, resins and asphaltenes. The latter, in turn, are more susceptible to biodegradation than resins and asphaltenes [47, 48]. Microbial Transformation and degradation of toxic organic chemicals. The spectra in figure 4 reveal the presence of aromatic compounds in the crudes treated with bacterial strains. Description of toluene inhibition of methyl bromide biodegradation in seawater and isolation of a marine toluene oxidizer that degrades methyl bromide.

Since hydrocarbon determinations were performed solely at the end of the study day 45no data are available to evaluate the biodegradation process in earlier time points. The organic phase of se samples was extracted with 45 mL of HPLC-grade dichloromethane three extractions with a volume of 15 mL each using the liquid-liquid method for 30 min in a separating funnel, filtering the obtained organic extract through anhydrous reagent grade sodium sulfate. No alkanes with backbones shorter than 12 carbon atoms were detected bactrias these samples.

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These microorganisms can be found not only in soil and water samples, but in clinical specimens, occasionally.

Hydrocarbon determinations Hydrocarbon determinations were performed at day Most research on bioremediation technology has focused therefore on aerobic heterotrophic bacteria, due not only to the taxonomic diversity of hydrocarbon-degrading representatives from this group, but to their ability to use xenobiotic compounds as carbon source in pure cultures [3].

Five strains belonging to the latter group could not be identified with the biochemical tests employed in this study. Monocyclic and polycyclic aromatic hydrocarbon fractions badterias analyzed by FTIR spectroscopy, as described in Materials and Methods. Based on our results, degrasadoras, together with the existing literature, it is possible to make some inferences.

Biodegradability of Venezuela crude oils.

The cultures were discarded after 15 days. Diversidad bacteriana en un biorreactor de lecho fluidificado durante el tratamiento de agua contaminada con nafta. Bioremediation of petroleum-and creosote-contaminated soils: Anaerobic microorganisms, however, are less versatile regarding their growth substrate and often display increased sensitivity toward heavy metals, hence playing a smaller role in biodegradation [2]. Comparative physiology of phenanthrene degradation by two dissimilar pseudomonads isolated from a creosote-contaminated soil.

Two genera of Gram-positive bacilli were identified Bacillus and Kurthiaas well as five genera of Gram-negative bacilli Alcaligenes, Acinetobacter, Marinomonas, Pseudomonas and Azotobacter.